Journal:

Article Title: Cerebromicrovascular endothelial cells are resistant to l -glutamate

doi: 10.1152/ajpregu.90430.2008

Figure Lengend Snippet: Effect of l-glutamate (l-glut) and NMDA on the viability of CMVECs and cortical neurons. A: viability of rat CMVECs was unchanged by replacing the culture medium with glucose-supplemented Earle's balanced salt solution (EBSS) for 8 h. Application of 1–10 mM l-glut or NMDA into the EBSS also did not decrease the viability of rat CMVEC cultures (n = 8–32). B: exposure of rat CMVEC cultures to 12-h oxygen-glucose deprivation (OGD) significantly reduced viability; however, addition of 1 mM l-glut or NMDA into the glucose-free EBSS solution did not affect cell death induced by OGD (n = 8–32). C: in contrast to CMVECs, in cortical neurons 1-h exposure to 0.2 mM l-glut or NMDA applied in the culture medium elicited significant cell death of up to 50% (n = 8–12). D: viability of CMVEC cultures derived from piglets showed no change after exposure to l-glut/NMDA (n = 16–32). *P < 0.05, significantly less than corresponding control values (open bars).

Article Snippet: Rat cerebral cortical neurons were isolated from embryonic day 18 Sprague-Dawley rat fetuses as described in detail elsewhere ( 23 ).

Techniques: Derivative Assay, Control